qPCR Testing

Real time qPCR is a rapid molecular method used to detect and quantitate Legionella pneumophila and Legionella species in potable and non-potable water.

If Legionella DNA is present it will be amplified and detected using fluorescence tagged to the Legionella gene. The concentration is compared to a standard curve and results are calculated and reported as low, moderate and high GU – Genomic Units. Genomic Units cannot be directly correlated to CFU because PCR reports live Legionella , dead Legionella and non-culturable Legionella .

Advantages:

  • Outbreak investigations
  • 6-48 hours turnaround time
  • No confirmation needed
  • Smaller sample size – 120 mL
  • Potable and Non-Potable water

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